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pSB2

价格:1200元

联系方式:I47-825O-882O

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载体基本信息

质粒名称: pSB2 
出品公司: Addgene
目录编号: 82026
存储实验室: Victor Sourjik

载体骨架基本信息
载体名称: pKG116
载体出品公司: --
载体抗性: 氯霉素
空载体大小: 4211 bp
完整质粒大小: 6087 bp
载体修饰: --
载体类型: --
筛选标记: --
高拷贝/低拷贝: 低拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: 编码大肠杆菌Tar和枯草杆菌McpC的杂交受体:McpC[1-286]-Tar[200-553]
物种来源: E. coli, B. subtilis
插入大小: 1923 bp
基因突变: --
GenBank编号 --
启动子 --
标签/融合蛋白: --
克隆方法: 限制性内切酶
5' 克隆位点: NdeI(未破坏)
3' 克隆位点: BamHI(未破坏)
5' 测序引物 CGGGCGCAATATTCATGTTG
3' 测序引物 --

文献引用方法
材料和方法部分: pSB2 was a gift from Victor Sourjik (Addgene plasmid # 82026 ; http://n2t.net/addgene:82026 ; RRID:Addgene_82026)
参考文献部分: Engineering Hybrid Chemotaxis Receptors in Bacteria. Bi S, Pollard AM, Yang Y, Jin F, Sourjik V. ACS Synth Biol. 2016 Jun 10. 10.1021/acssynbio.6b00053 PubMed 27285081

载体质粒图谱和多克隆位点信息

pSB2质粒图谱

载体简介

载体序列

LOCUS       pSB2                6087 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Hybrid McpC[1-286]-Tar[200-553] expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    pSB2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Bi S, Pollard AM, Yang Y, Jin F, Sourjik V
  TITLE     Engineering Hybrid Chemotaxis Receptors in Bacteria.
  JOURNAL   ACS Synth Biol. 2016 Jun 10.
  PUBMED    27285081
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      324..358
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(777..1436)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1351..1370
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1437..1539)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1931..1948)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2065..2610)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2145..2164)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgatcaagtc gcattggcgg tttcggaaat ggatcgcgtc acgcaacaga acgcatcgct
       61 ggtgcaggaa tcagctgccg ccgccgctgc gctggaagaa caggcgagtc gtttaacgca
      121 agcagtttcc gcgttccgtc tggcagccag cccactcacc aataaaccgc aaacaccatc
      181 ccgtcctgcc agtgagcaac caccggctca gccacgactg cgaattgctg aacaagatcc
      241 aaactgggaa acattttgag gatccgcgga taaataagta acgatccggt ccagtaatga
      301 cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg
      361 gtgagaatcg cagcaacttg tcgcgccaat cgagccatgt cgtcgtcaac gaccccccat
      421 tcaagaacag caagcagcat tgagaacttt ggaatccagt ccctcttcca cctgctgacc
      481 ggatcagcag tccccggaac atcgtagctg acgccttcgc gttgctcagt tgtccaaccc
      541 cggaaacggg aaaaagcaag ttttccccgc tcccggcgtt tcaataactg aaaaccatac
      601 tatttcacag tttaaatcac attaaacgac agtaatcccc gttgatttgt gcgccaacac
      661 agatccgtcg accgggtcga atttgctttc gaatttctgc cattcatccg cttattatca
      721 cttattcagg cgtagcaacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac
      781 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
      841 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
      901 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
      961 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
     1021 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     1081 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     1141 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     1201 tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     1261 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     1321 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     1381 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     1441 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     1501 tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag
     1561 ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga
     1621 tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg
     1681 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
     1741 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg
     1801 ctagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt
     1861 catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat
     1921 attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa
     1981 tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag
     2041 tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga
     2101 aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2161 tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
     2221 tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag
     2281 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat
     2341 ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag
     2401 ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact
     2461 gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg
     2521 gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa
     2581 gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat
     2641 ttctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga
     2701 tataagttgt aattctcatg tttgacagct tatcatcgat cccgcaagag gcccggcagt
     2761 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
     2821 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
     2881 ctaccgcatt aaagcttctt gcgaaacagc tctcgaccag gtttatactg gtcgtgcaga
     2941 tgaaagcagt ttttgccgag gtcgattccg accagcgcca tatcgctcat agtgatggtc
     3001 tccgaattat aaacaccctg cgaaagcgta gcctacgcag ggtgtcgggt gctcatccca
     3061 ttacccgttt tcccatctct taacttaagg ggggaacggt gtcctgtctt tcatggggct
     3121 cgttcactgc taacttacct gcttccctgt ctgtcaattc tctctatcct gcgatcccgc
     3181 gaagaaccaa aaaagctcga cagagggcgc ggtcatttta ggtcgggcgg atcggcgccg
     3241 ccggctcggc tggtgtgccg cacagcaccg cctacgtgag ctgccagttg atgaacttcc
     3301 cccgttgcca gctagggcgc aagcgggctg tataagatca ctgcccatca cattgatcgg
     3361 ctcggatttt ttctcaatcc gtaaacaggt caaacatcag ttgccgcaac caaatattgg
     3421 ctaggtcctt gtggtacttc gcatgccaga acatgttgat ggctatttca ggcaagacga
     3481 ctgggtgcgg caaggcgctt aggccgaagg gctccacaca gcagtcggct aaacgtatcg
     3541 gcacagtggc gagcagatcg gtgcgctgga ggatgtggcc aacggcggcg aagtgcggca
     3601 cttccagacg gatgtcgcgc cggatgccga cccgtgtcat gtacgtgtcc acctcgccgt
     3661 ggccggtgcc agcggcgatg acacgcacgt ggccgtagga acagaagcgc tccagagtca
     3721 ggggttcgcg ggtgactgga tggtccttgc gacataggca cacgtagtga ttctggagca
     3781 gccggcgctg aaagaagcca gtttgcagat tgggaagcag gcccacggcc aagtccacgg
     3841 ttccgttctg caaggcctgc atcaggctca tcgaactgtc gcgcaccgta ctgatcacgc
     3901 aattgggggc ctggtgagcc agcacatcca tcagccgcgg catgaagtag atctcgccaa
     3961 tgtcggtcat ggccagggtg aaggtacgct cgctggtcag cggatcgaag ctttcatggt
     4021 gctgtagggc gttgcgcagt gcgtgcatgg ccgaagtgac gggctcggcc agatgcgcgg
     4081 catagggtgt gggttccatt ccctgatgtg tgcgcacgaa gagtgggtcc tgtagcgagg
     4141 tgcgcaggcg tttcagcgca ttgctcacgg caggctgggt caggcccagg ttctccgcag
     4201 tgatagagac gcgtctgtcg accagcaact ggttgaacac caccagcagg tttaaatcca
     4261 ggtcacgcag ttccatgggg cctcgcttgg gttattgctg gtgcccggcc gggcgcaata
     4321 ttcatgttga tgatttatta tatatcgagt ggtgtattta tcaatattgt ttgctccgtt
     4381 atcgttatta acaagtcatc aataatgcca taaggagtac catatgttta aaaaacttca
     4441 catgaagatt gctgtttttg tttcgattat gttaatcatc acagttgttc tcttaatgct
     4501 gtcttcttat ttgacgctaa aaccgatgat tacagaggat gggaaaaata caactcaaaa
     4561 cgtgacgcag tcacttgaac aaaatattga attgcaatta aagagttatg ccatttcgtt
     4621 atcaagattg gctaatgggg aattaacgca tacatttgta acgaaaccaa gtaaagaagc
     4681 ttctcggctg tttcacgatg acattaagca aatcaaagac aacgatgatt atgttgcgat
     4741 ggcttacata ggcaccgcaa aaaaagaaat gtttacatat ccaaaagcgg atttcgctga
     4801 ggattacgat ccaacatcaa gaccatggta taagcttgca gctgaaaccc cggatcaagt
     4861 tgtttggacg gaaccttata aagatgtcgt gacaggagac atgattgtta ctgcatcgaa
     4921 agcgatctta gacaggcaaa aagtgatagg ggttgccagc tatgacttga aactctctgc
     4981 tatccagagt atggtcaata aacaaaaggt gccttacaaa ggatttgcct ttttagctga
     5041 tgcgagcggc aatcttttag cccatccgtc aaatcaagga aaaaatatat caaaggatca
     5101 aacactgcaa accatcgcat ccgagaaaaa agggatacaa gatgtgaatg ggaaaatggt
     5161 tgtgtaccaa acgattggcg agacaggctg gaaggtcgga acgcaatttg ataccgatca
     5221 attaatgtgg atttcagata aaatgaatcg ggccaatctt tggatatcat tgattgcgct
     5281 tgtggtggta ttgattctgc tggtggcgtg gtacggcatt cgccgtatgt tgcttactcc
     5341 gctggcaaaa attattgctc acattcgcga aatcgccggt ggtaacctgg cgaataccct
     5401 gaccattgac gggcgcagtg aaatgggcga cctggcgcag agcgtttcac acatgcaacg
     5461 ctctttgact gacaccgtca ctcatgtccg cgaaggttca gatgccatct atgccggtac
     5521 ccgtgaaatt gcggcgggca acaccgatct ttcctcccgt actgaacagc aggcatccgc
     5581 gctggaagaa actgccgcca gcatggagca gctcaccgcg acagtgaagc aaaacgccga
     5641 taacgcccgc caggcctcgc aactggcgca aagtgcctcc gacaccgccc agcacggcgg
     5701 caaagtggtg gatggcgtag tgaaaacgat gcatgagatc gccgatagtt cgaagaaaat
     5761 tgccgacatt atcagcgtta tcgacggtat tgccttccag actaatatcc tcgcgctgaa
     5821 tgccgcggtt gaagccgcgc gtgcgggtga acagggccgt ggttttgccg tggtggcggg
     5881 tgaagtgcgt aatcttgcca gtcgcagcgc ccaggcggca aaagagatca aagccctcat
     5941 tgaagactcc gtctcacgcg ttgataccgg ttcggtgctg gtcgaaagcg ccggggaaac
     6001 aatgaacaat atcgtcaatg ctgtcactcg cgtgactgac attatgggcg agattgcatc
     6061 ggcatcggat gaacagagcc gtggcat
//

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