血战棋牌

pREP10

价格:3000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pREP10
质粒类型: 哺乳动物表达载体;附加体载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: RSV
载体大小: 9287bp
5' 测序引物及序列: pREP Forward 5’ GCTCGATACAATAAACGCC 3’
3' 测序引物及序列: SV40pA rev 5'GAAATTTGTGATGCTATTGC'
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: Hygromycin
克隆菌株: DH5α
宿主细胞(系): 人类细胞;灵长类细胞;犬类细胞
备注:
pREP7载体是附加体型载体,可以在基因组外自主复制,类似的载体有:
   pREP4
   PCEP4
   pREP7
   pREP10
   pEBNA-DEST
   pEBVHisA
   pEBVHisB
   pEBVHisC
   pREP4/CAT
   pEBVHis-lacZ
 
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pREP10载体图谱



pREP10多克隆位点

载体简介

载体序列

LOCUS       Exported                9287 bp ds-DNA     circular SYN 12-AUG-2016DEFINITION  synthetic circular DNAKEYWORDS    pREP10  ORGANISM  synthetic DNA constructREFERENCE   1  (bases 1 to 9287)  AUTHORS   Biofeng Lab.FEATURES             Location/Qualifiers     source          1..9287                     /organism="synthetic DNA construct"                     /mol_type="other DNA"     polyA_signal    275..409                     /note="SV40 poly(A) signal"                     /note="SV40 polyadenylation signal"     promoter        complement(476..737)                     /note="RSV promoter"                     /note="Rous sarcoma virus enhancer/promoter"     polyA_signal    1503..1551                     /note="HSV TK poly(A) signal"                     /note="herpes simplex virus thymidine kinase                      polyadenylation signal (Cole and Stacy, 1985)"     CDS             complement(1593..2630)                     /codon_start=1                     /product="hygromycin B phosphotransferase"                     /note="HygR"                     /note="confers resistance to hygromycin"                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE                     MGEAN"     promoter        complement(2673..2818)                     /note="HSV TK promoter"                     /note="herpes simplex virus thymidine kinase promoter"     rep_origin      complement(3249..3837)                     /direction=LEFT                     /note="ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"     CDS             complement(4008..4868)                     /codon_start=1                     /gene="bla"                     /product="beta-lactamase"                     /note="AmpR"                     /note="confers resistance to ampicillin, carbenicillin, and                     related antibiotics"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS                     LIKHW"     promoter        complement(4869..4973)                     /gene="bla"                     /note="AmpR promoter"     rep_origin      7049..8837                     /note="oriP"                     /note="Epstein-Barr virus oriP replication origin (Yates et                     al., 2000)"ORIGIN        1 tctagagtcg acctcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga       61 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc      121 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagccgga tcataatcag      181 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa      241 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg      301 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc      361 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg gtaccagctg      421 aagcttgcta gcggccgctc gaggccggca aggccggatc cgtacccagc ttggaggtgc      481 acaccaatgt ggtgaatggt caaatggcgt ttattgtatc gagctaggca cttaaataca      541 atatctctgc aatgcggaat tcagtggttc gtccaatcca tgtcagaccc gtctgttgcc      601 ttcctaataa ggcacgatcg taccacctta cttccaccaa tcggcatgca cggtgctttt      661 tctctccttg taaggcatgt tgctaactca tcgttaccat gttgcaagac tacaagagta      721 ttgcataaga ctacatttcc ccctccctat gcaaaagcga aactactata tcctgagggg      781 actcctaacc gcgtacaacc gaagccccgc ttttcgccta aacacaccct agtcccctca      841 gatacgcgta tatctggccc gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag      901 attcttcatg caattgtcgg tcaagccttg ccttgttgta gcttaaattt tgctcgcgca      961 ctactcagcg acctccaaca cacaagcagg gagcagatac tggcttaact atgcggcatc     1021 agagcagatt gtactgagag tgcaccatac ggatctgcga tgataagctg tcaaacatga     1081 gaattggtcg actagcttgg cacgccagaa atccgcgcgg tggtttttgg gggtcggggg     1141 tgtttggcag ccacagacgc ccggtgttcg tgtcgcgcca gtacatgcgg tccatgccca     1201 ggccatccaa aaaccatggg tctgtctgct cagtccagtc gtggacctga ccccacgcaa     1261 cgcccaaaat aataaccccc acgaaccata aaccattccc catgggggac cccgtcccta     1321 acccacgggg ccagtggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg     1381 gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc     1441 ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt     1501 atgaacaaac gacccaacac ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc     1561 gggttccttc cggtattgtc tccttccgtg tttcagttag cctcccccat ctcccgatcc     1621 ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc     1681 cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg     1741 acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag     1801 ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct     1861 gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac     1921 ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc     1981 cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc     2041 gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga     2101 gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg     2161 ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt     2221 ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc     2281 tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca     2341 ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc     2401 acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag     2461 aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg     2521 aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt     2581 tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat atctcattgc     2641 cccccgggat ctgcggcacg ctgttgacgc tgttaagcgg gtcgctgcag ggtcgctcgg     2701 tgttcgaggc cacacgcgtc accttaatat gcgaagtgga cctcggaccg cgccgccccg     2761 actgcatctg cgtgttcgaa ttcgccaatg acaagacgct gggcggggtt tgtgtcatca     2821 tagaactaaa gacatgcaaa tatatttctt ccggggacac cgccagcaaa cgcgagcaac     2881 gggccacggg gatgaagcag ggcggcacct cgctaacgga ttcaccactc caagaattgg     2941 agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc     3001 catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcggggccga cgcgctgggc     3061 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc     3121 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac     3181 gaccatcagg gacagcttca aggccagcaa aaggccagga accgtaaaaa ggccgcgttg     3241 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt     3301 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc     3361 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct     3421 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc     3481 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta     3541 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca     3601 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag     3661 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag     3721 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt     3781 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa     3841 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg     3901 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga     3961 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta     4021 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc     4081 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg     4141 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga     4201 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt     4261 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt     4321 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc     4381 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc     4441 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca     4501 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag     4561 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg     4621 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa     4681 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa     4741 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga     4801 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga     4861 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg     4921 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt     4981 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa     5041 aataggcgta tcacgaggcc ctttcgtctt caagaattct catgtttgac agcttatcat     5101 cgataagctg atcctcacag gccgcaccca gcttttcttc cgttgcccca gtagcatctc     5161 tgtctggtga ccttgaagag gaagaggagg ggtcccgaga atccccatcc ctaccgtcca     5221 gcaaaaaggg ggacgaggaa tttgaggcct ggcttgaggc tcaggacgca aatcttgagg     5281 atgttcagcg ggagttttcc gggctgcgag taattggtga tgaggacgag gatggttcgg     5341 aggatgggga attttcagac ctggatctgt ctgacagcga ccatgaaggg gatgagggtg     5401 ggggggctgt tggagggggc aggagtctgc actccctgta ttcactgagc gtcgtctaat     5461 aaagatgtct attgatctct tttagtgtga atcatgtctg acgaggggcc aggtacagga     5521 cctggaaatg gcctaggaga gaagggagac acatctggac cagaaggctc cggcggcagt     5581 ggacctcaaa gaagaggggg tgataaccat ggacgaggac ggggaagagg acgaggacga     5641 ggaggcggaa gaccaggagc cccgggcggc tcaggatcag ggccaagaca tagagatggt     5701 gtccggagac cccaaaaacg tccaagttgc attggctgca aagggaccca cggtggaaca     5761 ggagcaggag caggagcggg aggggcagga gcaggaggtg gaggccgggg tcgaggaggc     5821 agtggaggcc ggggtcgagg aggtagtgga ggccggggtc gaggaggtag tggaggccgc     5881 cggggtagag gacgtgaaag agccaggggg ggaagtcgtg aaagagccag ggggagaggt     5941 cgtggacgtg gagaaaagag gcccaggagt cccagtagtc agtcatcatc atccgggtct     6001 ccaccgcgca ggccccctcc aggtagaagg ccatttttcc accctgtagg ggaagccgat     6061 tattttgaat accaccaaga aggtggccca gatggtgagc ctgacgtgcc cccgggagcg     6121 atagagcagg gccccgcaga tgacccagga gaaggcccaa gcactggacc ccggggtcag     6181 ggtgatggag gcaggcgcaa aaaaggaggg tggtttggaa agcatcgtgg tcaaggaggt     6241 tccaacccga aatttgagaa cattgcagaa ggtttaagag ctctcctggc taggagtcac     6301 gtagaaagga ctaccgacga aggaacttgg gtcgccggtg tgttcgtata tggaggtagt     6361 aagacctccc tttacaacct aaggcgagga actgcccttg ctattccaca atgtcgtctt     6421 acaccattga gtcgtctccc ctttggaatg gcccctggac ccggcccaca acctggcccg     6481 ctaagggagt ccattgtctg ttatttcatg gtctttttac aaactcatat atttgctgag     6541 gttttgaagg atgcgattaa ggaccttgtt atgacaaagc ccgctcctac ctgcaatatc     6601 agggtgactg tgtgcagctt tgacgatgga gtagatttgc ctccctggtt tccacctatg     6661 gtggaagggg ctgccgcgga gggtgatgac ggagatgacg gagatgaagg aggtgatgga     6721 gatgagggtg aggaagggca ggagtgatgt aacttgttag gagacgccct caatcgtatt     6781 aaaagccgtg tattcccccg cactaaagaa taaatcccca gtagacatca tgcgtgctgt     6841 tggtgtattt ctggccatct gtcttgtcac cattttcgtc ctcccaacat ggggcaattg     6901 ggcataccca tgttgtcacg tcactcagct ccgcgctcaa caccttctcg cgttggaaaa     6961 cattagcgac atttacctgg tgagcaatca gacatgcgac ggctttagcc tggcctcctt     7021 aaattcacct aagaatggga gcaaccagca ggaaaaggac aagcagcgaa aattcacgcc     7081 cccttgggag gtggcggcat atgcaaagga tagcactccc actctactac tgggtatcat     7141 atgctgactg tatatgcatg aggatagcat atgctacccg gatacagatt aggatagcat     7201 atactaccca gatatagatt aggatagcat atgctaccca gatatagatt aggatagcct     7261 atgctaccca gatataaatt aggatagcat atactaccca gatatagatt aggatagcat     7321 atgctaccca gatatagatt aggatagcct atgctaccca gatatagatt aggatagcat     7381 atgctaccca gatatagatt aggatagcat atgctatcca gatatttggg tagtatatgc     7441 tacccagata taaattagga tagcatatac taccctaatc tctattagga tagcatatgc     7501 tacccggata cagattagga tagcatatac tacccagata tagattagga tagcatatgc     7561 tacccagata tagattagga tagcctatgc tacccagata taaattagga tagcatatac     7621 tacccagata tagattagga tagcatatgc tacccagata tagattagga tagcctatgc     7681 tacccagata tagattagga tagcatatgc tatccagata tttgggtagt atatgctacc     7741 catggcaaca ttagcccacc gtgctctcag cgacctcgtg aatatgagga ccaacaaccc     7801 tgtgcttggc gctcaggcgc aagtgtgtgt aatttgtcct ccagatcgca gcaatcgcgc     7861 ccctatcttg gcccgcccac ctacttatgc aggtattccc cggggtgcca ttagtggttt     7921 tgtgggcaag tggtttgacc gcagtggtta gcggggttac aatcagccaa gttattacac     7981 ccttatttta cagtccaaaa ccgcagggcg gcgtgtgggg gctgacgcgt gcccccactc     8041 cacaatttca aaaaaaagag tggccacttg tctttgttta tgggccccat tggcgtggag     8101 ccccgtttaa ttttcggggg tgttagagac aaccagtgga gtccgctgct gtcggcgtcc     8161 actctctttc cccttgttac aaatagagtg taacaacatg gttcacctgt cttggtccct     8221 gcctgggaca catcttaata accccagtat catattgcac taggattatg tgttgcccat     8281 agccataaat tcgtgtgaga tggacatcca gtctttacgg cttgtcccca ccccatggat     8341 ttctattgtt aaagatattc agaatgtttc attcctacac tagtatttat tgcccaaggg     8401 gtttgtgagg gttatattgg tgtcatagca caatgccacc actgaacccc ccgtccaaat     8461 tttattctgg gggcgtcacc tgaaaccttg ttttcgagca cctcacatac accttactgt     8521 tcacaactca gcagttattc tattagctaa acgaaggaga atgaagaagc aggcgaagat     8581 tcaggagagt tcactgcccg ctccttgatc ttcagccact gcccttgtga ctaaaatggt     8641 tcactaccct cgtggaatcc tgaccccatg taaataaaac cgtgacagct catggggtgg     8701 gagatatcgc tgttccttag gaccctttta ctaaccctaa ttcgatagca tatgcttccc     8761 ttgggtaaca tatgctattg aattagggtt agtctggata gtatatacta ctacccggga     8821 agcatatgct acccgtttag ggttaacaag ggggccttat aaacactatt gctaatgccc     8881 tcttgagggt ccgcttatcg gtagctacac aggcccctct gattgacgtt ggtgtagcct     8941 cccgtagtct tcctgggccc ctgggaggta catgtccccc agcattggtg taagagcttc     9001 agccaagagt tacacataaa ggcaatgttg tgttgcagtc cacagactgc aaagtctgct     9061 ccaggatgaa agccactcag tgttggcaaa tgtgcacatc catttataag gatgtcaact     9121 acagtcagag aacccctttg tgtttggtcc ccccccgtgt cacatgtgga acagggccca     9181 gttggcaagt tgtaccaacc aactgaaggg attacatgca ctgccccgcg aagaaggggc     9241 agagatgtcg tagtcaggtt tagttcgtcc ggggcgggga tcgatcc//

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