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pET-3d(+)

价格:800元

联系方式:I47-825O-882O

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pET3d载体基本信息

出品公司: Novagen
载体名称: pET-3d(+)
质粒类型: 大肠杆菌载体
克隆菌株: DH5α
启动子: T7
终止子: T7 terminator
载体大小: 4637bp
5' 测序引物及序列: T7:TAATACGACTCACTATAGGG
3' 测序引物及序列: T7-ter:TGCTAGTTATTGCTCAGCGG
载体标签: N-T7
载体抗性: 氨苄青霉素
克隆菌株: DH5α 
表达宿主: BL21(DE3)
培养条件: 37℃,有氧,LB
诱导方式: 无需诱导
组成型: 组成型

pET3d载体质粒图谱和多克隆位点信息

pET-3a载体图谱

pET3d载体简介

pET-3a-d载体携带一个N端t7•tag序列和BamH I克隆位点。这些 载体是许多pET家族载体的前身;pET-23a-d(+)系列对应于 PET-3A-D,但包含几个附加功能。独特的地点显示在圆形地图上。 注意,序列由pbr322约定编号,因此t7表达式区域是 在图谱上反转。转录的编码链的克隆/表达区 T7 RNA聚合酶如下所示。PET-3A-D载体携带一个N末端T7•Tag®序列和BAMH I克隆位点。这些 载体是许多pet家族载体的前身;pet3d系列对应于 PET-3A-D,但包含几个附加功能。独特的地点显示在圆形地图上。 注意,序列由pbr322约定编号,因此t7表达式区域是 在图谱上反转。

pET3d载体序列

LOCUS       pET-3d(+)                4637 bp ds-DNA     circular SYN 25-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pET-3d
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, August 25, 2016 from SnapGene Viewer 3.1.4
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..19
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             80..112
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /note="T7 tag (gene 10 leader)"
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     terminator      178..225
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(591..619)
                     /note="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        732..836
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             837..1697
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1868..2456
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    2642..2784
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2886..3077)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
       61 tttaagaagg agatatacca tggctagcat gactggtgga cagcaaatgg gtcggatccg
      121 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      181 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      241 atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc
      301 aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac
      361 gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc
      421 gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc
      481 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt
      541 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt
      601 atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc
      661 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      721 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      781 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      841 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      901 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      961 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1021 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     1081 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1141 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1201 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1261 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1321 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1381 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1441 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1501 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1561 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1621 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1681 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1741 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1801 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1861 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1921 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1981 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     2041 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2101 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2161 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2221 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2281 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2341 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2401 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2461 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2521 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2581 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     2641 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg
     2701 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     2761 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     2821 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     2881 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
     2941 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
     3001 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     3061 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
     3121 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
     3181 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
     3241 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
     3301 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
     3361 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
     3421 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
     3481 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc
     3541 cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac
     3601 gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga
     3661 ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg
     3721 tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg
     3781 cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg
     3841 tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa
     3901 gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc
     3961 cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga
     4021 acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct
     4081 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc
     4141 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga
     4201 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca
     4261 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
     4321 ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     4381 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     4441 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     4501 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     4561 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     4621 tctcgatccc gcgaaat
//

      

pET3d载体质粒图谱pdf版和相关资料下载

pET3d载体应用举例

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